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High production of poly(3-hydroxybutyrate) from a wild Bacillus megaterium Bolivian strain

机译:从野生巨大芽孢杆菌玻利维亚菌株高产量的聚(3-羟基丁酸酯)

摘要

Taking into account that a novel strain ofudBacillus megateriumudwasudisolated from Uyuni salt lake (Bolivia) in a previous work, the objectives ofudthis new study were to determine the maximal Poly-3-hydroxybutyrateudproduction potential ofudB. megateriumudstrain uyuni S29 in an industrialudconventional media, the possibility that the strain accumulates different typesudof polyhydroxyalkanoates, the cellular morphology during the biosynthesisudprocess and the characterization of the produced biopolymers.udMethods and Results:udThe micro-organism was first tested in a 3-L bioreactorudobtaining a high specific growth rate of 1ud64 hud1ud. A second fed-batch experimentudwas carried out in shaking flasks, reaching up to 70% PHB of cell dry mass. Theudbiosynthesized polymers were extracted by two different extraction proceduresudand characterized. The results showed that all of them were PHB with thermaludproperties different to the conventional PHB. The micrographs taken by TEMudshow the different cell morphology during the fermentation process.udConclusions:udIn this previous study, the strain not only grew properly in theudindustrial conditions proposed without spore formation, but also producedudand accumulated a large content of PHB, never reached before for its genus.udTherefore, if the culture conditions can be optimized, the biopolymerudproduction could be increased.udSignificance and Impact of the Study:udThe impact of the study has related toudthe area of the biomaterials and their production. The study provides new dataudrelated to the high production of PHB from the wild novel strainudB. megateriumuduyuni S29, the highest polymer accumulation for the genusudBacillusudwithout spores formation.
机译:考虑到以前在玻利维亚的乌尤尼盐湖中发现了一种新的 udbacillus megaterium udud udus分离株,因此该新研究的目的是确定 udB的最大Poly-3-hydroxybutyrate ud生产潜力。在工业非常规培养基中产生的巨型细菌 udstrain uyuni S29,该菌株可能会积聚不同类型的 udud聚羟基链烷酸酯,在生物合成 ud过程中的细胞形态以及所产生的生物聚合物的表征。 ud方法与结果: ud微生物首先在3-L生物反应器中测试伪装获得1 ud64 h ud1 ud的高比生长速率。在摇瓶中进行了第二次补料分批实验,达到了细胞干重的PHB高达70%。通过两种不同的提取程序提取 udbio合成的聚合物,并对其进行了表征。结果表明,它们都是PHB,其热性能与常规PHB不同。透射电镜拍摄的显微照片 ud显示了发酵过程中不同的细胞形态。 ud结论: ud在以前的研究中,该菌株不仅在建议的工业条件下没有孢子形成而正常生长,而且产生 ud并积累了大量的成分。 ud因此,如果可以优化培养条件,则可以提高生物聚合物 ud产量。 ud研究的意义和影响: ud研究的影响与 b的面积有关生物材料及其生产。该研究提供了与野生新菌株高产PHB有关的新数据 udB。 megaterium uduyuni S29,没有芽孢形成的 udBacillus ud属的最高聚合物积累。

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